Electrochemical Determination of 2-thiouracil in Pharmaceuticals and Real Samples Using Gold Electrode

In this study, the electrochemical behaviour of 2-thiouracil (2TU) has been investigated using cyclic, differential pulse and square wave voltammetric techniques. The electrochemical studies were carried out in pH 3 with 0.2 M phosphate buffer as supporting electrolyte. Cyclic voltammetric studies were performed in a wide range of sweep rates and various concentrations of 2TU. The effect of surfactants was studied. The anodic peak was characterized and process was adsorption-controlled. According to the linear relation between the peak current and the 2TU concentration, differential-pulse voltammetric method for the quantitative determination in pharmaceuticals was developed. The linear response was obtained in the range of 1–11 μM with a detection limit of 2.05×10-8 M with good selectivity and sensitivity. The proposed method was successfully applied to 2TU determination in pharmaceutical samples and for the detection of 2TU in urine as a real sample.