Study of Stability, Zeta-potential, and Steady Rheological Properties of Nanoliposomes Containing Vitamin D3

Vitamin D is a group of sterol compounds and a liposoluble vitamin that has major role in the matrix of cartilage and bones. Food fortification with liposoluble vitamin is not straightforward, because of this vitamins is poorly dispersible in aqueous systems such as beverages. The encapsulation of nutraceutical compounds in lipid-based carrier systems, ¬like nanoliposomes, is effective in the preservation of their native properties during storage. The objective of the present study was to prepare vitamin D3 nanoliposomes, characterization, and stability studies of prepared formulation. In this experimental study, the nanoliposomes containing vitamin D3 were prepared from -various quantities of lecithin and cholesterol (60-0), (50-10), (40-20), (30-30) by thin-film hydration¬-sonication method. Various testes such as zeta-potential, turbidity and rheology were carried out to determine physicochemical properties of liposomes. The physical stability of the prepared formulations, evaluated by measuring the average particle size and the remaining vitamin D3 in nanoliposomes during storage, over 30 days at 4¬˚C. Zeta-potential results showed that inclusion of cholesterol in liposome caused to increase of negative zeta-potential from -29 to -42.9 mv. The samples containing cholesterol showed no significant changes in mean diameter volume and the amount of vitamin loaded liposome, during this period. The incorporation of cholesterol into the phospholipid bilayer increased the turbidity. All liposome samples showed Newtonian behavior at all concentration of lecithin- cholesterol. The applied method in this study has been efficient method for encapsulation of vitamin D3, reduction of size in the nonometric range, a narrow size distribution, physical stability and else properties.