The Study of Molecular and Population Diversity of Rutilus frisii kutum in Anzali Lagoon and Shirud River by Using of Molecular Genetic Method

Anzali Lagoon and Shirud River are two important spawning areas in south Caspian Sea for kutum. Due to the economically valuable of this fish, molecular and population genetic structure of this fish was investigated using microsatellite markers. For this purpose, 100 samples from two regions were collected and DNA was extracted. Ten pairs microsatellite primer was used for PCR which all made polymorphic patterns. Data resulted: 191 alleles were observed. The maximum numbers of alleles (18) were found in two loci (Ca1 and C 3) and the minimum number of alleles (2) was found in MFW1 locus. The differences between samples of two regions were not statistically significant (P>0.05) neither for average number of alleles per locus nor for observed heterozygosity. The calculated Fst was 0.056 and significant (P< 0.01). Most samples of two areas were not at Hardy-Weinberg equation test. The genetic distance was 0.407 which indicating the high genetic distance between populations. The data generated in this study showed that the Caspian Sea kutum in Anzali Lagoon and Shirud River are two independent populations. Therefor preservation of gene diversity and implementation of fisheries management based on excess of each population should be considered.