Evaluation of Biochemical Parameters in Sheep after Placement of Tissue Engineered Valve in Thoracic Aorta

Valvulardiseases are a common problem and valvular replacement is usual treatment for valvular destruction. Tissue engineered heart valve has some advantages, that make them an interesting alternative for previous valves. the aim of this study is measurement of biochemical factors for evaluation of short term efficacy of tissue engineered heart valve. This study was undertaken on 5 male sheep with average weight of 45-55 kg. They have been of-feed 24 hours before of operation. The tissue engineered heart valves were placed in thoracic aorta and after surgery they connected to ventilator under SIMV mode. We took arterial blood sample every hours for 12 hours and evaluated, PCO2, PO2, tCO2, SO2, Hct, Na+ and K+. Arterial blood sample has been taken from auricularis artery. Blood pH fell down in all sheep after surgery but in two sheep as a result of sever blood loos during surgery pH went up toward acidosis, finally after 12 hours pH in all sheep was in normal range. After operation PCO2 was low an PO2 was high because of mechanical ventilation. All the factors backed to normal range after 12 hours of operation. this study shows biochemical factors had fluctuation in first 12 hours but after 12 hours most of them backed to normal range.