The Effect of -Adrenergic Agonist Isoproterenol on miR-886-3p and miR-23a Expression in Human Bone Marrow Mesenchymal Stem Cells

Mobilization of Hematopoietic Stem Cells (HSCs) for transplantation and the importance of -adrenergic signals in induction of this process have been well investigated. However، little is known about the role of -adrenergic signals in mobilization of HSCs and factors influenced by these signals. The Chemokine Stromal Derived Factor -1 (SDF-1) which is expressed by human bone marrow-derived mesenchymal stem cells (hMSCs)، has a key role in mobilization of HSCs. In addition، miR-886-3p and miR-23a can regulate the expression of SDF-1 in hMSCs. In this study، to investigate the role of miR-886-3p and miR-23a in mobilization process، expression of both miRNAs was evaluated in hMSCs treated by Isoproterenol (a -adrenergic agonist). hMSCs were isolated from human bone marrow and cultured. Following flowcytometric analysis، the cells were treated with 100 M isoproterenol. Total RNA was extracted at 12 and 48 hours post treatment، and also from untreated hMSCs as control. Then، miR-886-3p and miR-23a expression levels were quantified by quantitative Reverse Transcriptase PCR. The expression level of miR-886-3p increased significantly at 12 and 48 hours post treatment (P<0. 05). In addition، the expression level of miR-23a decreased at 12 hours post treatment and increased significantly at 48 hours post treatment (P<0. 05). Isoproterenol induces miR-886-3p in hMSCs. MiR-23a is primarily decreased، and then increased due to treating with isoproterenol. So both miRNAs can contribute to mobilization process. References 1- Horowitz MM، Gale RP، Sondel PM، et al. Graft-versus-leukemia reactions after bone marrow transplantation. Blood.