Occurance and Detection of Bean Yellow Mosaic Virus In Faba Bean Fields of Kerman Province

Faba bean is an important legume in Iran and many other countries. Bean yellow mosaic virus (BYMV) has been found in faba bean fields in a number of countries. In this survey، during 2010 growing season، a total of 240 faba bean samples with mosaic، distortion and stunting symptoms suspected for BYMV infection were collected from faba bean fields of Jiroft، Kerman province. These samples were analyzed using BYMV polyclonal antibody in serological tests (DAS-ELISA and TPIA). In faba bean fields of Jiroft، the percentage of infection with BYMV was 62. 5. Among the collected weeds، only two samples of Chenopodium quinoa were infected by BYMV. In greenhouse mechanical inoculation assays، the virus induced local lesions and systemic symptoms on a number of plant species of Chenopodiaceae، Amaranthaceae، Fabaceae، Solanaceae and Brassicaceae families. A fragment of 1700 nt length from the NIb، CP and 3´-UTR regions of a BYMV isolate (J2) was amplified in IC-RT-PCR using a Potyviridae universal primer pairs. BLAST analysis on the partial NIb region of the isolate and comparison of the nucleotide sequence showed that this isolate is similar to a Japanese isolate. This is the first report of occurrence of BYMV in faba bean fields of Kerman province.