Evaluation antibacterial activity of Zataria multiflora Boiss. essential oil and Zein bioactive lm

With the aim of using plant essential oils (EOs) as natural antibacterial in the bioactive zein film, the chemical composition of Zataria multiflora Boiss. was analyzed by GC–MS. Minimum inhibitory concentration and minimum bactericidal concentration of Zataria multiflora EOswere evaluated against for Listeria monocytogenes and Escherchia coli by using the method of micro-well dilution and head space phase. Zein based films containing 5 and 10 % of Zataria multiflora Boiss. (ZEO) were developed. The antimicrobial activity of the films was investigated in a vapor phase and liquid media. Carvacrol and thymol were detected as the major components. MICand MBC were obtained from above components against for Listeria monocytogenes)500 µg/l and 1000 respectively(compared to Escherchia coli were more effective. Listeria monocytogenes was more sensitive to ZEO on vapors phase. A highest inhibition zone was observed against for Listeria monocytogenes by incorporating 10% (v/v) ZEO in the vapor phase. zein film containing 5% ZEO had no inhibitory ability on bacteria in addition to in concentration of 10% just reduced the density of bacteria, Log reduction value of bioactive film of zein against Listeria monocytogenes and Escherchia coli were increased the direct-contact. These results revealed that ZEO as natural food additive has good potential to be incorporated into zein to make antimicrobial for food applications.