Identification BF2*21 and BF2*13 alleles of Major Histocompatibility Complex in two Native Chickens, Khazak and Dashtiari

The aim of this research was to study two alleles BF2*13، BF2*21 of BF2 gene in Class I MHC in two population at Sistan and Baluchestan Province. MHC is a gene cluster that play regulation role in immune system. Many experiments showed association between the gene cluster and resistance and sensitiveness against different diseases and، also productivity traits. BF2 gene is among associated loci. In the research، blood samples were collected randomly from 72 native chickens (37 Khazak and 35 Dashtiari chickens). After DNA extraction from blood samples with standard kit، the quantitative and quality of DNA were determined by gel agarose 1%. Next stage would be designing appropriate primers for BF2*13 and BF2*21 alleles. Using PCR this alleles were amplified and PCR product observed by gel electrophoresis which were 52bp and 197 bp، respectively. Frequency of samples contain BF2*13 allele in two populations Khazak and Dashtiari observed 75. 6 and 22. 8%، respectively. Also frequencies of samples contain BF2*21 allele in two populations Khazak and Dashtiari were 45. 9 and 34. 2%، respectively. In other hand، in Khazak population that frequency samples contain BF2*13 alleles is high، allele frequency samples contain BF2*21 allele that associated with resistance to Marek disease and in flu and IBD and some productive traits، is relative property، then animal breeders could be use allele frequency of these alleles in breeding programming.