Improvement of doublehaploids production with colchicines in rice anther culture media

A study was set up in the research station of rice research institute of iran (RRII) to rate plant double haploids anther culture-derived plant F1. Anther cultures of a genotype to put on two medium Chu، N6 were treated with or without (0،20،40،60 mg/l) colchicines for 7 days and then the anthers from culture media containing colchicines transfer to medium without colchicines. The effect of media، colchicines concentration and their intractions on callus iniation، regeneration and double haploidy، the compelet random factorial design with three replication was formed. The results indicated to be affected significantly between callus induction medium for four manners case evaluation (callus induction، green plants، total regeneration) but not to be between on callus induction media for double haploidy affected significantly. Also means comparison at P= 0/05 based on Duncan،s multiple range test، indicated culture media N6 (5/102) was considerably better for callus induction and for green plants، total regeneration and double haploidy. The most percentage of double haploids were in 60 mg/l colchicine concentration (68/19) that with other concentrations was doubling rate control 0 mg/l (31/98) affected significantly. Also callus induction media Chu with 60 mg/l concentration (69/17) and callus induction media N6 with 60 mg/l concentration 67/22) had the most double haploids. culture media N6 was the least with control 0 mg/l colchicines (30/00). In fact of to be between different concentration in callus induction media affect significantly.