Study of gene expression pattern in Citrus grandis plants infected by Candidatus Liberibacter asiaticus causal agent of citrus greening disease
Author(s):
Abstract:
Citrus greening has been reported from south of Iran in 2007. The molecular basis of compatibility and disease development in this system is poorly understood. We have carried out a cDNA-AFLP analysis to identify resistance genes of Citrus grandis and Candidatus Leiberibacter aciaticus susceptibility genes in late infection development stage. Selective amplifications with 10 primer combinations allowed the visualization of about 25 transcript-derived fragments (TDFs) in inoculated leaves, which were differentially expressed. We sequenced 16 fragments, which were identified as Citrus grandis transcripts after homology searching, while 5 were homologous to sequences in NCBI databases and were attributed to Candidatus Leiberibacter asiaticus. Many Citrus grandis genes spanning almost all functional categories were upregulated during infection. This study provides the first global catalogue of Citrus grandis and Candidatus Leiberibacter asiaticus genes expressed during inoculation, together with their functional annotations. This will help to elucidate the molecular basis of the resistance process and identify genes and chemicals that could help to inhibit the pathogen.
Keywords:
Language:
Persian
Published:
Applied Entomology and Phytopathology, Volume:83 Issue: 1, 2015
Pages:
15 to 26
magiran.com/p1427914
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!