Bioaccumulation of some bioenergetics resources in Sunn pest, Eurygaster integriceps (Hem. Scutelleridae), under treatment of Neem-azal
Stored bioenergetic resources in overwintering adults of sunn pest, Eurygaster integriceps, is important in their hibernation. The present study examined some biochemical changes in the adults treated by sub-lethal concentrations (≤ LC30) of Neem-azal T/S. Initially, adults of new generation were sprayed by different concentrations of Neem-azal (100, 300, 500 and 0 µl/l) under field conditions for 3, 6 and 12 days. Then, the adults of the first experiments were resprayed a week later by Neem-azal at the same concentrations prior to resampling for 3, 6 and 12 days. The amounts of lipid, sugar, glycogen and protein of four males and females were determined (mg/g; w/w). Results revealed significant effects Neem-azal on total lipid, glycogen, sugar, protein and energy content (P< 0.001). So that all of three concentration increased lipid and protein contents versus control hemipterans. The net amount of lipids and proteins were increased in 100, 300 and 500 µl/l levels respectively 85.53%, 97.3%, 108.22% and 4.62%, 14.23%, 34.27%. The glycogen quantity decreased 27.35% in 100 µl/l concentration level and no significant differences in other doses with respect to control. Also sugar quantity was decreased 9.9, 27.37 and 43.76% respectively in 100, 300 and 500 µl/l concentration levels. The energy content (EC) was increased in three doses and this trend conntinued with the passage of time. Respraying also was affected on increasing of EC and adult sexes not significant effect on it.
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