Studies on the Antioxidant and Antimicrobial Activity and Flavonoid Derivatives from Fruits of Trigonosciadium brachytaenium (Boiss.) Alava.

The objective of this study was to evaluate the potential use of the fruits of Trigonosciadium brachytaenium in the pharmaceutical and food industries. Flavonoids are present in food and medicinal plants and are thus consumed by humans. They are found in plants as glycosides. Their biological activities have an impact on human health so that they serve as target molecules to develop new drugs. From methanolic extract of fruits of Trigonosciadium brachytaenium (Boiss.) Alava. (Umbelliferae family), two flavonoid derivatives namely 5- Hydroxy- 3’-methoxy-4’- ethoxyflavone-7-O (’’-(4’’’- acetyl-rha)-rha)(1) and 5- Hydroxy- 4’-methoxy-8- ethoxyflavone-7-O-(2’’-(2’’’- acetyl-rha) rha)(2) have been isolated by column chromatography (CC) and preparative TLC (PTLC). Those structures were elucidated by UV, 1H- and 13C- NMR, HMBC, EI-MS and IR spectra. The antioxidant activity of methanol extract was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method. The results indicate that methanol extract from aerial parts of T. brachytaenium possess considerable antioxidant activity. The highest radical scavenging activity was detected (IC50 = 47 μg/mL). This study reveals that the methanolic extract of this plant is attractive sources of flavonoid, especially the essential ones, as well as of effective natural antioxidants. The antimicrobial activity of the methanol extract of aerial part was determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassay showed that the extract exhibited good antimicrobial activity. These flavonoid compounds were isolated for the first time from T. brachytaenium.