Effects of Cobalt Nano Particles on Involved Genes Expression in Sesamin Biosynthesis Pathway in Sesamum indicum L.

The aim of this study was to investigation cobalt nano-particles effects on expression level of key genes involved in sesamin biosynthetic pathway including CYP81Q1، CYP81Q2، CYP81Q3 and C3H in Sesamum indicum L. cell suspension culture. First sesame cell suspension culture was established. For this purpose، Karaj1 hypocotyle cultivar، 0. 6 mgL-1 BAP، 3 mgL-1 NAA were used. Different concentrations of cobalt nano-particles elicitor including 0. 25، 0. 5 and 1 mgL-1 were added to the 18 days-old culture and sampling was carried out after 2، 8 and 24 hours post treatment. Expression level of mentioned genes was measured by the Real-Time qRT-PCR technique. Data analysis and figures depiction were done using Excel. Results showed that MS medium consisting of 0. 6 mgL-1 BAP، 3 mgL-1 NAA and 30 gL-1 socrose، 26ºC، 130rpm and darkness condition are optimum situation for condensed sesame suspension production. Adding cobalt nano-particles to the suspension culture، led to increasing genes expression level، especially، at 0. 5 mgL-1 elicitor concentration in different times. Since this elicitor had no negative effects on the mentioned genes expression، the effect of higher concentrations on these genes expression can be studied in the future. Negative effects of this elicitor on the expression of genes involved in sesamin biosynthesis means decreasing sesamin content. Based on obtained results elicitation of sesame cell suspension culture by cobalt nano-particles led to the increasing genes expression involved in the sesamin biosynthetic pathway. Therefore، this elicitor can be used to induce higher expression levels of these genes.