Effect of temperature, life stage and soil drainages of host and non-host plants on hatching of cabbage cyst nematode, Heterodera cruciferae

Stimulation of egg hatching of cabbage cyst nematode (Heterodera cruciferae) were investigated by soil drainages of host (kohlrabi and white cabbage) and non-host (lettuce) plants in different life stages, under different temperature conditions including 5, 10, 15, 20 and 25°C. Soil drainages of non-host plant and three and six-months-old seedlings of host plants were collected at 2, 4, 6 and 8 leaves developmental stages. Different concentrations of soil drainagesincluding undiluted soil drainages, soil drainagesdiluted 1:4 or 1:16 with water were used. Pot’s draining water and tap water were used as two negative controls and 0.3 M ZnCl2 solution as a positive control. Twenty-five cysts were stored in a 24-well plastic plate containing the soil drainagessolutions. The rate of eggs hatching was noted with a week intervals up to three weeks. The results showed that dilution of six-months-old white cabbage soil drainages1:4 at six leaves stage enhanced stimulation of hatching at 20°C compared with other treatments.