Molecular detection of mutations causing drug resistance mycobacterium tuberculosis strains among patients with tuberculosis in Mazandaran

Drug resistance Tuberculosis is a serious problem in treatment procedure of patients with Tuberculosis. Rapid diagnosis of the drug resistance Tuberculosis is very important in controlling the progress of the disease. The aim of this study was to identify the mutations responsible for drug resistant among affected patients in Mazandaran using LPA method. Fifty four samples of Tuberculosis patients that were registered at health centers in Mazandaran were randomly selected and after sputum culture on Lowenstein–Jensen medium, genomic DNA was extracted from colonies using CTAB method. For molecular analysis of mutations causing resistance to 5 different drugs, Long Probe Assay (LPA) method using GenID kit was applied. Of the 54 investigated cases 11(including 9 female and 2male) had at least one mutation causing antibiotic resistance strain. Five cases had resistance to more than one drug. None of the patients were affected with multiple drug resistance Tuberculosis, so MDR Tuberculosis is not a serious threat in Mazandaran. However, LPA is a fast method for detecting drug resistance Tuberculosis and using this approach is recommended for all patients with Tuberculosis.