Mapping genomic regions controlling some physiological traits associated with salt tolerance in recombinant inbred lines of wheat

In order to determine the genomic regions of some physiological traits of "Seri M82×Babax" derived of 167 recombinent inbred line population of wheat under normal and salinity stress conditions, an experiment was conducted in two randomized alfa lattice design with two replications. Traits such as prolin content, water soluble carbohydrate, relative water content, chlorophyll a, chlorophyll b, chlorophyll c, Na+, K+ and Na+/K+ rario were measurerd. QTL analysis was carried out using genetic linkage map derived from 475 AFLP, SSR and DArT markers with total length of 1440.4 and the average distance of 3.03 cM and QTL cartographer 2.5 software with composite interval mapping method. Combined analysis of variance showed significant differences between lines for all studied traits. Maximum correlation was between chlorophyll b and chlorophyll c traits. In general, 23 QTLs were found for studied traits (10 and 13 QTLs for normal and stress conditions, respectively). Phenotypic variances that were explained by these QTLs changed from 6.80 to 15.65. The highest and lowest phenotypic variances were found for Na+/K+ ratio and chlorophyll c in the stress condition. LOD scores were ranged from 3.10 to 7.87. The highest and lowest LOD scores were attained for Na+/K+ ratio and water soluble carbohydrate in the stress condition. Q6ARWC of QTL controlling leaf relative water content, Q3APro controlling proline content, Q6AK controlling potassium concentration, Q4ANa/K controlling sodium to potassium ratio in different environments studied were present and had the necessary stability. So they might be used in marker assisted selection programs. Marker assisted selection for this trait may increase the resistance of plants to maintain growth in soils exposed to salt stress at critical stages of its growth.