The Effect Of 6 Weeks Endurance Training On BDNF Expression In Sensory Spinal Cord Of Rats With Diabetic Neuropathy

The aim of present study was to investigate the effect of 6 weeks of endurance training on gene expression of Brain Derived Neurotrophic Factor (BDNF) sensory roots of sciatic nerve in rats with diabetic neuropathy. Twenty-eight adult male Wistar rats in the body mass range of 326/3±8/4 gr, randomly assigned in to four groups: diabetic control, diabetic training, healthy control and healthy training. For inducing diabetic neuropathy, after twelve hours of food starvation, intraperitoneal injection of STZ solution (45 mg/Kg) method was used. Two weeks after STZ injection, the endurance training protocol was performed for six weeks and Twenty-four hours after the last training session, rats were sacrificed. Real-Time PCR was used for BDNF gene expression measurement. For data Analysis, Kruskal– Wallis and Mann-Whitney U Test were used (P≤0.05). Results indicate that diabetes decreases BDNF expression (10-folds) in sensory roots of sciatic nerve. However, 6 weeks of endurance training can partly compensate the BDNF expression decrease induced by diabetic neuropathy (P=0.043). This study shows that diabetes can decrease BDNF expression in sensory roots of sciatic nerve. however, this decrease can be reversible some deal by endurance. In summary, result of this study support this hypothesis that neurotrophic support decreases in diabetic neuropathy.