Evaluation of Modification in M2 gene of Influenza H1N1 virus in Expression of it in BL21 Ecoli

Aim and By Attention to gene sequence and each amino acid codon in host which express protein, also in order to probable vaccine production against influenza A, a major cause of mortality in a year,this research was carried out. M2 protein is conserved and it can be a proper candidate vaccine for influenza infection.
Present research was an experimental study. The sequence of M2 protein of Iran influenza virus H1N1 strain was modified and synthesized. It was subcloned into pET22b vector recombinant protein and expressed in BL21 Ecoli and confirmed with specific antibody by western blot technique.
The modified M2 gene was expressed in Ecoli. The concentration of it was 300μg/ml and it was confirmed by western blotting with specific antibody.
Influenza M2 protein with modification in sequence could be expressed in BL21 Ecoli host.