Study of different sulfur concentrations on Alliinase gene expression profile in some garlic (Allium sativum L.) clones with RT-Real time PCR

Apot culture experiment was carried out to assess the effect of 6 sulfur concentrations (0, 6, 12, 18, 24 and 60 g) on alliinase gene expression in 3 Iranian garlic clones on randomized block designed in 3 replications. Results based on Real-time quantitative PCR indicated the responses of garlic cultivars could be reflected as changes in gene transcripts but all gene expression patterns revealed no positive and continuously correlation. Gene expression decreased in sulfur supply conditions (6 to 18 g) firstly and then (18 to 24 g) increased intensively. At very high concentration (60 g) restate it reaches its minimum value. The findings of this research can be used in further research on the garlic metabolic engineering of active ingredients and also reducing or enhancing the activity of the responsible genes for garlic pungency.