Correlation of necrosis with angiogenesis and proliferation in heterotopic xenograft model of breast cancer

Breast cancer is considered as one of the most important malignancies in women. Each year, a considerable population of women dies from this disease. To date, basic and clinical cancer research has been developed. The most portions of these researches have been devoted to the breast cancer. All new anticancer drugs must successfully pass preclinical in vitro and in vivo assays to enter clinical trials. To assay the validity of heterotopic xenograft models of breast cancer from the viewpoint of correlation of necrosis with angiogenesis and proliferation index. BT-474 cell line of breast cancer was inoculated heterotopically and bilaterally to 10, 6-8 week old athymic nude mice. Severity of necrosis was determined by H&E staining. Angiogenesis and proliferation index were calculated from immunohistochemistry slides stained by CD34 and Ki-67, respectively. Then, the correlation of necrosis with angiogenesis and proliferation was assessed. There was seen a positive correlation between necrosis and proliferation (p