The comparison of self-renewal gene expression pattern in cancer cell lines, tumor tissue and normal tissue of the bladder tumor

Re-expression of self-renewal genes is one of the causes of cancer. So, expressional profile of self-renewal genes including, OCT4, NANOG, KLF4, SOX2 and Nucleostemin in two cell lines (5637 and HT1376), cancer and normal tissues of five bladder cancer biopsy tissues were analyzed.
In this experimental study, the cell lines were cultured in RPMI1640 medium. Cancer tissue samples were selected from samples referred to the pathology laboratory, according to clinical symptoms and laboratory findings. Tissue edges were selected as healthy tissue. Expressional profile of interested genes (mRNA expression) was carried down by Real-Time PCR, data were analyzed by ∆∆ct formula and expressional rate reported as fold changes.
The Real-Time PCR results showed that the expression of studied genes in cancer cell lines and tumor tissues were down by similar pattern but did not expressed in healthy tissue.
The expression of OCT4, NANOG, KLF4, SOX2 and Nucleostemin genes were necessary for induction of self-renewal potency. The results showed that these genes were expressed in cancer tissue and cancer cell lines in compared to normal tissues. The investigation of re-expression of mentioned genes is recommended.