Evaluation of cytotoxic properties of Aqueous and alcoholic extracts of liquorice plant (Glycyrihiza glabra L.) on DU-145 cell line

Prostate cancer is the second most common cancer in men after skin cancer. Throughout the years, medicinal plants, in some cases is the only treatment were considered. Glycyrihiza glabra L. liquorice plant with the scientific name 'Glycyrihiza glabra L.' which belongs to the family Fabaceae, there are three species of perennial herbaceous plants in Iran. Root extract is widely used in medicine, food industry, Tobacco and other industries throughout the world. In this study, the cytotoxic effect of aqueous and hydroalcoholic extracts of licorice root on human prostate cancer (DU145) with the MTT assay was studied. Licorice root through Agriculture and Natural Resources Research Center of Isfahan was prepared. Next the aqueous and hydroalcoholic (ethanol) extracts of the plant was prepared. The cell category of DU-145 in the Cultivation enviroment RPMI-1640 Containing 10% bovine serum in incubator were cultured with 5% CO2 and in different concentrations of the aqueous and Hydroalcoholic (ethanol) during 24, 48, 72 hours was incubated. The method of MTT for calculating the survival rate of the cells were incubated in the presence and the Lack extracts were used and Light absorption at 540 nm was read by ELISA. Data were evaluated using SPSS software (N=12, *=P-value