Evaluation of Culture and Human Fibroblast Cells Transplant (Allograft) on Amniotic Membrane for Treatment of Epidermplysis Bullosa

Introduction & Epidermplysis bullosa (EB) is a genetic disease with skin fragility and instability at the junction of dermis to epidermis. Severe deformity of hands because of scars and adhesions leads to lose the proper function of hand which affects the quality of patients’ life. Covering the wound after opening adhesion is the major problem in the way of reconstructive surgeons. Our purpose in this study is to use allogeneic fibroblasts amnion after surgery as a cover in patients to prevent further adhesion.
This study was an experimental study that was done on six Epidermplysis bullosa Patients with hand deformity and Adhesion. We took a skin sample from back of one of parents’ ear. Then fibroblast was separated, cultured and transferred to the amniotic membrane. After separating the full adhesion, all parts without skin were covered with the allogeneic fibroblast amnion. Furthermore, speed, quality, recovery time of wound were examined as well as range of motion in finger joints and the pressure on skin.
The results of the study showed that wound healing well done and for wound healing varies between 15 to 29 days and the average length of treatment was 1.23 days with a standard deviation 77.3. Skin repair and rehabilitation activities as well as the pressure of splint has endured.
Allogeneic fibroblast with a scaffold like amnion can reduce the need for skin graft in patients with Epidermolysis Bullosa. Normal allogeneic fibroblast and disability of releasing collagen seven in these patients can be effective factors in wound healing and be better than autogenic fibroblasts.