The effect of stem cell factor on proliferation of human endometrial CD146+ cells

Stem cell factor (SCF) is a transcriptional factor which plays crucial roles in normal proliferation, differentiation and survival in a range of stem cells.

The aim of the present study was to examine the proliferation effect of different concentrations of SCF on expansion of human endometrial CD146 cells.

In this experimental study, total populations of isolated human endometrial suspensions after fourth passage were isolated by magnetic activated cell sorting (MACS) into CD146 cells. Human endometrial CD146 cells were karyotyped and tested for the effect of SCF on proliferation of CD146 cells, then different concentrations of 0, 12.5, 25, 50 and 100 ng/ml was carried out and mitogens-stimulated endometrial CD146 cells proliferation was assessed by MTT assay.

Chromosomal analysis showed a normal metaphase spread and 46XX karyotype. The proliferation rate of endometrial CD146P P cells in the presence of 0, 12.5, 25, 50 and 100 ng/ml SCF were 0.945±0.094, 0.962±0.151, 0.988±0.028, 1.679±0.012 and 1.129±0.145 respectively. There was a significant increase in stem/ stromal cell proliferation following in vitro treatment by 50 ng/ml than other concentrations of SCF (p=0.01).

The present study suggests that SCF could have effect on the proliferation and cell survival of human endometrial CD146P cells and it has important implications for medical sciences and cell therapies.