Fabrication of Nafion/Silver Nanoparticles/Reduced Graphene Nanosheets /Glucose Oxidase Nanobiocomposite for Electrochemical Glucose Biosensing

The aim of this work was fabrication of a nanobiocomposite as a smart biosensor for monitoring glucose. A glucose nanobiosensor based on silver nanoparticles (SNs) and reduced graphene nanosheets (rGNs) composite was successfully fabricated and developed. The SNs-rGNs nanocomposites were successfully prepared and coated on the surface of strip. The reduced graphene nanosheets (rGNs) support incorporated to zero-dimensional Ag nanoparticles arrayed in the third dimension amended a glucose biosensor with superior sensing characteristics. The sensor abilities were certified by changing sensor performance (i.e., sensitivity, limit of detection, and linear range) through using a novel nanocomposite. This approach to fabricate a biosensing device qualifies a high sensitive biosensor design that demonstrated outstanding biological sensing performance such as boosted glucose sensitivity (32 nM limit of detection, 14.1 mAM−1cm−2 sensitivity, 200 nM to 9 mM linear sensing range), a long stable shelf-life (>21 days), and a high selectivity. To the best our knowledge, this device is the first biosensor protocol for glucose monitoring.