Invitro Anti-Bacterial and Anti-Oxidative Activity of GlycyrrhizaGlabra L. from North of Golestan Province

Glycyrrhizaglabra L. is one of the most widely used
medicinal herbs in Golestan province that is known for its anti-inflammatory, carminative,
antiviral, anti-infection and anti-ulcer properties in Iranian traditional medicine. This study
aimed to assess the anti-bacterial and anti-oxidative activity of G. glabrafrom the Golestan
province.
The rip root of the plant was collected in autumn 2013. The ethanolic extract of the plant was prepared by maceration method. The anti-oxidative property of the plant was assessed by total antioxidant capacity (TAC), reducing power (RP) and 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity assays. The anti-bacterial activity was assessed using agar-well diffusion method and the minimum inhibitory concentration (MIC) assay.
The ethanolic extract of G. glabrahad relatively high anti-oxidative activity with IC50 value of 130 μg/ml, especially in the DPPH method. The extract also exhibited high anti-bacterial activity against the following Gram-positive bacteria: Staphylococcus aureus (21.1±0.7 mm), Staphylococcus epidermidis (19.6±0.2 mm), Bacillus subtilus (19.3±0.6 mm), followed by Escherichia coli (12.1±0.8 mm), Enterococcus faecalis (13.2±0.1 mm) and Kelebsiellapneumoniae (11.5±0.4 mm) with MIC values in the range of 31 - 132 mg. mL-1.
According to results, the root extract of G.glabrais a good source of
antioxidant compounds with suitable anti-bacterial activity, which can be used as natural
anti-infection and anti-inflammatory agent for treatment of many diseases.