Effect of sonication on the size of growth hormone transfersome

The transfersomes are ultraflexible vesicles¡ which due to their elastomechanic properties are able to penetrate into pores smaller than their own size¡ and thereby have the potential to deliver drugs to the skin. In this study growth hormone transfersomes were constructed from two formula made of phospholipids¡ sodium deoxycholate (f1) and sodium lauryl sulphate (f2) as surfactants. There are different ways of obtaining transfersomes with a specified size range. One of the methods is the use of sonication to regulate their size and dimensions. Accordingly¡ in order to study transfersomes carrying the growth hormone¡ the vesicles were sonicated using both the probe and bath methods. In each case¡ dimensions¡ dispersity¡ the zeta potential of transfersomes and biological activity of the growth hormone were measured during three different time cycles of sonication. The results showed that during investigation of all the different time periods¡ growth hormone activity was found to decrease further following probe sonication when compared to that of bath sonication. Therefore¡ vesicle size and zeta potential were measured in the probe sonication tests. The mean size was found to vary between 242.6 to 190.6 and 81.37 to 159.6¡ for the f1 and f2 vesicles¡ respectively. The zeta potential of the f1 transfersomes was more negative than that of f2.