Evaluation of gibberellin and auxin production ability by endophytic bacteria Pseudomonas putida in soybean TMS cultivar

Endophytic bacteria live inside tissues of their plant host without causing visible symptoms, and in more cases are reported as plant growth promoting bacteria. This study was conducted to determine plant growth promoting affects of endophytic bacteria associated with Soybean (cultivar TMS).
In order to isolate endophytic bacteria from various parts of soybean (cultivar TMS), samples were collected from the farm of Islamic Azad University of Sanandaj. After genomic DNA extraction, 16S rDNA gene was amplified using PCR. Then, the PCR product was sequenced by BLAST. Strains were surveyed for IAA, and GA production ability was carried out using a randomized complete design in three replications.
The isolated bacteria were able to produce IAA in various amount 3.2 -12.14 µg/ml without tryptophan and in the presence of it, 7.2 -15.14 µg/ml. GA producing abilities were also 0.11-0.20 µg/ml for these isolates. Based on the 16S rDNA sequence, the isolated bacteria was belonged to Pseudomonas putida with 99% similarity.
This study is the first report of isolation of P. putida from Soybean (TMS cultivar). The endophytic bacteria isolated in this study can be used to promote plant growth.