Effect of Fusarium graminearum cell wall on lignans and phenolic compounds in cell culture of Linum album

Linum album Kotschy ex Boiss., one of endemic species in Iran, produces podophyllotoxin (PTOX) and other lignans. Podophyllotoxin which occurs in a few plant species and has pharmacological significance for it's anticancer activities. Manipulation of cell culture media by elicitors is one of the important strategies for inducing secondary metabolism and production of valuable metabolites. In this study, first we isolated cell wall of Fusarium graminearumas described by Di Pietro et al. (2009). We investigated the different concentrations effect of fungal cell wall on cell growth and PTOX and lariciresinol production in cell suspension cultures of L. album. Then, the optimal concentration of fungal cell wall (1% v/v) was selected for further experiments and thus after 7 days of pre-culture, the cells were treated with 1 % (v/v) of cell wall. Lignan analysis with HPLC demonstrated that production of podophyllotoxin and lariciresinol significantly increased. Also phenolic compounds have reached the most accumulation at 5 day after treatment. But flavnoids and flavones had increased at 3 and 7 day after treatment. As defensive compound, lignin increased after treatment.