Evaluation of serum calcium, phosphorus, alkaline phosphatase, magnesium, total protein and albumin after ovariectomy in the rat

Osteoporosis is a systemic disease characterized by decrease in bone mass and micro architectural deterioration of bone tissue. In this study, in order to evaluate the serum concentration of calcium, phosphorus, magnesium, alkaline phosphatase, total protein and albumin during osteoporosis, 80 female Sprague-Dawley rats with approximate age of 10 weeks were allocated to 10 groups (3 control, 3 sham, 3 treatment groups and one pure control group). The animals in treatment groups were ovariectomized under general anesthesia whereas only the abdominal wall was incised in the sham groups using the same approach of the treatment groups. Treatment groups and shams were maintained for 5, 12 and 21 weeks respectively after ovariectomy alongside related control groups. Blood samples were collected from the retroorbital sinus at the end of each stage for measurement of the blood parameters. Evaluation of the serum levels of calcium, phosphorus and alkaline phosphatase revealed statistically significant differences between the ovariectomized groups and control groups and also pure control group and sham groups. The highest level of calcium and phosphorus was observed 5 weeks after ovariectomy and the lowest level at 12 and 21 weeks after ovariectomy. The levels of alkaline phosphatase had increased at 5, 12 and 21 weeks after ovariectomy. Statistically the mean levels of magnesium, albumin and total protein did not reveal a significant difference between treatment groups, shams and pure control group.