Application Periodical Bioreactor in Micropropagation of Bayoder Cultivar of Gerbera jamesonii Boluse ex Hooker

Unlike conventional micropropagation method¡ using bioreactors in large scale results in low production costs. Capitulum explants were grown in solid media¡ there after¡ regenerated shoots were transfered to Modified MS medium supplemented with different levels of BAP2¡ BAP (0.5¡ 1¡ 2¡ 3¡ 4 mg l-1) in combination with 0.1 mg l-1 IAA was used for proliferation stage in solid media in complatly randomized design with 4 replications. The results showed that BAP at concentration of 4 mg l-1in combination with 0.1 mg l-1 IAA caused the highest multiplication rate in solid medium. Multiplication rate in temporary immersion bioreactor (TIB) system was done as complatly randomized dosign with 3 replications with different times of immersion (2¡ 4 and 6 times daily¡ each time lasted 2 minutes)¡ supplemented with IAA (0.1 mg l-1) and BAP (0.5¡ 2 and 4 mg l-1)¡ and also in MMS solid medium supplemented with IAA (0.1 mg l-1) and BAP (0/5¡ 1¡ 2¡ 3 and 4 mg l-1). The result showed that 2 times of immersion had the highest shoot quality¡ but 4 times treatment showed the highest average number of regenerated shoots (25.1)¡ leaf number (8.06)¡ plantlet height (3.8) and fresh weight of regenerated mass (7.06)¡ and was considered as the best treatment of immersion in TIB. In TIB 2 mg l-1 BAP in combination with 0.1 mg l-1IAA had the highest amount of multiplication rate¡ and 0.5 mg l-1BAP in combination with 0.1 mg l-1IAA showed the best shoot quality than in solid medium. Overall¡ TIB could be used as an efficient and economically viable system in micropropagation of Gerbera.