Antibiotic-Loaded PLGA Nanoparticles against Pseudomonas aeruginosa

Pseudomonas aeruginosa biofilm is of interest due to its involvement cystic fibrosis and prosthesis-related infections. Unique biofilm structure, the vast application of antibiotics and accordingly antibiotic resistance, increase the need for new drug delivery methods to enhance drug efficiency. Nanocarriers are one of the new choices. The aim of the presented study is to evaluate the efficiency of amikacin-loaded PLGA nanoparticles in P. aeruginosa biofilm eradication.
Antibacterial (MIC and MBC) and antibiofilm (MBEC) activity of synthesized nanoparticle, by double emulsification method, was assayed against P. aeruginosa PAO1.
The synthesized nanoparticles contain an appropriate amount of antibiotic )≈26 μg Amikacin/mg/ nanoparticle ) and have a suitable size )≈440 nm) for inhalation and injection. The particles are sustainable, have a good antibiotic release kinetic for antibacterial applications. Furthurmore, the antibiotic loaded in the particles also keep their antibacterial activity.
Amikacin is known to possess nephrotoxic and ototoxic properties and therefore loading it in a polymeric cover, makes the nanoparticles suitable candidate antibacterial and antibiofilm applications with fewer side effects.