Saturation of barley genetic map using retrotransposon-based markers and doubled haploid population derived from a cross of Clipper×Sahara

Genetic maps with high density and wide genome coverage play significant role in basic and applied genetic researches. In recent decades, with the advent of DNA markers, the huge development has been created in genetic map preparation and saturation in various plant species. In this study, IRAP, REMAP and ISSR markers were applied to saturate the genetic map of barley in a population with 149 double haploid individuals derived from a cross of Clipper and Sahara. The basic genetic map of this population was used as a frame for further analysis. In general, among the 120 primers used, 6 IRAP, 7 REMAP and 3 ISSR primers showed polymorphism between parents which generated 11 IRAP, 7 REMAP and 4 ISSR markers. Eighteen markers segregated in population, out of which 12 markers assigned to 7 barley linkage groups. Two markers deviated from the ratio of 1:1. The maximum number of markers was assigned to linkage group 2. In the current investigation, retrotransposon-based markers were able to saturate the gaps of barley genetic map on linkage groups 1, 3, 5 and 6. The results showed that retrotransposon markers can be effectively used for saturation of barley genetic maps.