Manganese Effect on Gene Expression of Flavones Synthetize and Flavonoids Amounts in Cuminum Cyminum

The flavones are sub-group of flavonoids with wide varieties. Also they protect plants against UV, also have a role in treating human diseases. The test has been performed in a randomized complete block design with 3 replications for expression of flavone synthetize gene (FNS1) and flavonoids compounds in cumin. 18-day-old cumin seedlings were treated. They were harvested in times of 1, 2, 3 and 4 days after the stress treatment of manganese. FNS1 gene expression and composition of flavonoids and anthocyanin were measured by RT-PCR and spectrophotometer respectively. The results showed that the highest amount of gene expression, flavonoids amounts and anthocyanin were observed at 2nd day after manganese stress. The results suggests that the content of FNS1 expression, flavonoids and anthocyanin, especially during the 2 days of stress, after oxidative stress which resulting from the absorption of the active element of the system of antioxidant plant