The Survey of the Nano Polyamidoamine - G5 (NPAMAM-G5) Dendrimer Antibacterial Properties on Bacillus Subtilis, Salmonella Typhi, Shigella Dysenteriae and Escherichia Coli from aqueous solution

Dendrimers are a subset of branched structures that have certain structural order. The aim of this study was to investigate the performance of Nano Polyamidoamine–G5 (NPAMAM-G5) dendrimers in removal of Escherichia Coli, Salmonella Typhi, Shigella Dysenteriae and Bacillus Subtilis from aqueous solution.
Material and In this experimental study, initially dilution of 103 CFU/ml were prepared from each strain of bacteria. Then different concentrations of dendrimers (0.025, 0.25, 2.5 and 25 µg/ml) in the ambient temperature (23-25° C) was added to water. In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 50 and 60 min) and were cultured on nutrient agar medium. Samples were incubated for 24 hours at 37° C and then number of colonies were counted.
Antibacterial properties of dendrimers in aqueous solution by increasing the dendrimer concentration and contact time, increased. At a concentration of 25 μg/ml and 60 minutes all kinds of bacteria except Shigella Dysenteriae (98% removal efficiency) 100% removal was observed. The concentration of 2.5 μg/ml at 60 minutes of bacteria, Escherichia Coli and Bacillus Subtilis,100% Were removed. All concentrations of dendrimer at different times were reduced bacteria.
Results of this study indicated that NPAMAM–G5 dendrimer is able to remove Escherichia Coli, Salmonella Typhi, Shigella Dysenteriae and Bacillus Subtilis in aqueous solution.