Determination of CGRP Role in Lipid Oxidation During Endurance Exercise in Male Wistar Rats

Energy homeostasis during exercise is a complex interaction of neuronal and hormonal inputs that are integrated at the level of the central nervous system (CNS). The aim of the present study was to investigate the role of calcitonin gene related peptide (CGRP) in fat oxidation during acute exercise in male Wistar rats. Thirty animals were randomly divided into three groups: control (C; n = 10), training (T; n = 10) and training CGRP (8-37) (Tࡤ37; n = 10). The animals from T and Tࡤ37 groups performed a single bout of endurance exercise at 26 m/min for 60 min. Cerebrospinal fluid (CSF) and blood samples were collected immediately after exercise .The animals from Tࡤ37 group received CGRP (8-37) (an antagonist of CGRP receptor, 10 μg/kg; IP) before endurance exercise. CGRP concentrations in the cerebrospinal fluid and serum, FFA, and triglyceride concentrations were measured by ELISA. The difference between groups were evaluated with one-way ANOVA test and Tukey’s post-hoc test. CGRP concentrations in the cerebrospinal fluid and serum were significantly higher in the animals from T (P