Frequency vat1 and fimC genes in isolates of Escherichia coli tissue of patients with colorectal cancer and inflammatory bowel disease large Iranian population

Aim and Colorectal cancer is the third most common cancer after lung cancer, stomach and liver cancer in the world. Studies have shown that patients with inflammatory disease of the large intestine are at high risk of developing colorectal cancer are located. Several factors make inflammatory bowel disease and colorectal cancer is one of the factors involved, bacteria and toxins derived from them. Research shows that some strains of E. coli can be induced to increase the mutation rate that was established in 2010 by Cuvas-Romas and colleagues. fimC and vat1 Virulence genes in these organisms play an important role in bacteria pathogenicity. This study is to compare the virulence genes in E .coli strains isolated from samples biopsy patient's inflammatory bowel disease and colorectal cancer, intestinal tissue was performed.
38 biopsies were obtained from intestinal tissue and E.coli bacteria in microbial and biochemical method was identified and isolated. After DNA extraction, strains for virulence genes vat1, fimC using polymerase chain reaction (PCR), were evaluated.
Molecular analysis showed a significant difference between the groups studied the gene vat1 (p = 0.0245) and 42.8% of positive samples were for this gene in normal groups, 87.5% with inflammatory bowel disease87.5 % of patients with colorectal cancer were reported. But there is no significant difference between the groups in terms of fimC gene (P = 0.201). And 71.4% of positive samples for this gene in normal group's and 93.7% in patients with inflammatory bowel disease73.3 % of patients with colorectal cancer were reported.
The results, to confirm the good relationship between virulence genes studied induced inflammation and proliferation by inducing mutation rate.