Biosurfactant Production by Pseudomonas putida1694 in Various Carbon Sources

Bio-surfactants as surface active compounds are produced by microorganisms. In this study bio-surfactant production by Pseudomonas putida1694 were evaluated in different carbon sources (kerosene, sugar cane molasses and glucose) at 30 and 37 °C within the incubation periods of 48 and 168 hours. For screening bio-surfactant production, hemolysis, and oil collapse and emulsification index, surface tension reduction and cell surface hydrophobicity tests were done. Results showed that P. putida1694 was able to grow in all carbon sources. Based on bio-surfactant production, this strain had a positive or β hemolysison blood agar medium. The Compounds produced by this strain in each of carbon sources at both temperatures and incubation periods collapsed down. The maximum surface tension reduction was recorded in the samples containing kerosene as carbon source incubated at 37 ° C for 168 hours, in which bacterium reduced surface tension to 20.33 mNm-1. Themaximum emulsifying and cell hydrophobicitywere recorded in molasses (28%) and kerosene(70%) respectively. Bio-surfactant production amounts by P. putida1694 in kerosene, glucose and molasses as carbon sources were 10.2, 1.7 and 5.5 g L-1, respectively. Due to higher production of bio-surfactant in the presence of kerosene, it seems that the bacteria can be used for refining oil-contaminated soils.