A Simple Method for Determination of Nicotinic Acid in Urine Samples by using Differential Pulse Voltammetry and Carbon Paste Electrode

A new electrochemical method was introduced for nicotinic acid measurement in pharmaceutical samples. Electrochemical behavior of nicotinic acid was studied by cyclic voltammetry and differential pulse voltammetry on carbon paste electrode. Some important parameters affecting the electrode response to nicotinic acid was investigated and optimized. The optimal electroanalysis pH was found to be 2.2. Moreover, the optimal amount of binder used for carbon paste preparation was observed to be 27.50% of total mass of the paste composite. The peak current response versus nicotinic acid concentration was linear in the range of 5.0×10−5 to 3.0×10−3 M. The detection limit was calculated to be 3.1×10−5 M. The developed method was successfully used for determination of nicotinic acid in the urine samples.