Identification of single nucleotide polymorphisms of PPP2CA gene and its effect on carcass, growth and fat storage traits in Lori Bakhtiari and Zel sheep

The purpose of this study was to determine the polymorphism of PPP2CA gene in chromosome 5 of sheep and its effect on carcass, growth and fat storage traits in Lori-Bakhtiari and Zel sheep. Blood samples were collected from 140 Zel sheep (breeding or slaughtering), and 165 Lori-Bakhtiari sheep, and their DNA was extracted. Then, the fragment of 243 bp of exon 1 of PPP2CA gene was amplified of and its various patterns were sequenced. Two different banding patterns were observed and the results of sequencing showed existence of two mutations. Within the group of breeding Zel and Lori-Bakhtiari, the effect of T/T and Del/Del genotypes on weaning weight (27.79±1.46 vs 30.67 ± 1.57) and cholesterol (60.76±0.94 vs 54.26±1.65) was significant (P