Influence of Adipose Derived Mesenchymal Stem Cells on the Effective Inflammatory Factors of Diabetic Wound Healing in Animal Models

Background and Chronic non-healing diabetic wounds are a major medical challenge worldwide. Stem cell therapy has been developed as a new approach in the management of such wounds that need to be explored further. In the present study we aimed to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) on gene expression level of some important inflammatory factors involved in wound healing in diabetic animal model.
ADSCs were isolated from adipose tissue of mice and their stemness was identified in terms of specific cell surface markers (CD44, CD90, and CD34) and multi-lineage differentiation potentials. Then 106 stem cells were injected in dermis area around the wounds and the wound tissues were tested for the expression level of IL-8 and IL-10 genes and histopathological analysis at 3rd, 7th, 14th, and 21st days after treatment.
Expression level of IL-10 gene at days 3, 14 and 21 increased significantly (P The present study indicated that stem cells could improve the indicators of wound healing.