Evaluation of Histopathological and Histomorphological Effects of Kombucha Extract (Camellia sinenesis) on Stomach Surgical Wound Healing in Adult Male Rat

Wounds healing especially as to surgical wounds is considered clinical problem. Kombucha is used as a food and adminstarted for wound healing. The aim of this study was to assess histopathological and histomorphometrical effect of Kombucha on surgical wound healing process in stomach of rat.
Thirty adult male Wistar rats with 10-week-old and weighted between 200-250 g were randomly divided into two groups (Control and Experimenral). After anesthesia, rats’ stomach was removed and a gastrotomy incision with the length of 1 cm was made in greater curvature, thereafter, sutured in 2 layers. Experimental group received Kombucha extract (1 ml/100gbw) and control group received normal saline (1 ml/100gbw) daily for 14 days through the gavage. In 3, 7 and 14 days after operation, five rats of each group were euthanized and stomach tissue specimens were collected for histopatholocical study by H&E and Masson's trichrome staining. Histopathological and histomorphometrical studies of wound healing among the groups were carried out considering more effective agents on wound healing including proliferation of fibroblasts, angiogenesis, re-epithelialization and collagen organization in healing tissue.
The numbers of fibroblasts, capillary buds and organized collagen content in healing site of experimental group were significantly more than control group (p Based on the results, Kombucha extract possesses beneficial effects on healing of stomach surgical wounds in rats.