The effect of folate supplementation during 10 weeks of resistance training on serum and stomach level of ghrelin and serum level of insulin in male Wistar rats

The purpose of this study was to investigate the effect of folate supplementation during 10 weeks of resistance training on serum and stomach level of ghrelin and serum level of insulin in male Wistar rats. Thirty two male Wistar rats (8 weeks old, 204.4±18.2 g.BW) after 1 week familiarization and based on their weights, were randomly divided into four equal groups (8 rats in each group): control, folate supplementation, resistance training, resistance닪榕黖궊 groups. Training groups carried out 10 weeks (5 session/week) of resistance training on 1 meter height ladder (divided by 26 stairs) with load corresponding to 30℅ of their body weight (suspended from tail) at the first week and was gradually increased to 250℅ at the last week. Supplementation was conducted by dissolving 10 mg folic acid in per liter of consumed water. Serum concentrations of ghrelin and insulin and stomach concentrations of ghrelin were assessed using ELISA method. The results showed that serum level of ghrelin in resistance group decreased significantly in compare with control (P=0.002), supplementation (P=0.001) and resistance닪榕黖궊 (P=0.017) groups. Also the ghrelin of stomach increased significantly in resistance닪榕黖궊 group in compare with control (P=0.014) and resistance (P=0.017) groups. There was no significant differences between 4 groups in serum insulin (P=0.673). Based on our results, folate supplementation during resistance training increases the ghrelin of stomach or at least prevents from decreases the serum ghrelin.