Alterations of gyrA in Enterobacteriaceae isolated of urinary tract ‎infection resistance to quinolones antibiotics

Urinary tract infection is one of the most common infections, which if not treated, it can ýcause serious problems in patients. One of the ways to treat of this infection is antibiotic therapy. ýNowaday, antibiotic resistance in microorganisms is a main problem for physicians and patients in the ýworld. The aim of this study was to evaluate the genetic resistance to quinolones antibiotics in ýEnterobacteriaceae isolated in urine samples. ý
100 bacteria of Enterobacteriaceae family were isolated from suspected ýsamples of urinary infection. Antibiotic susceptibility of isolated bacteria to quinolone antibiotics, ýincluding ciprofloxacin, nalidixic acid, norfloxacin, ofloxacin, levofloxacin and enrofloaxin, was ýperformed by disc diffusion method according to standard guidelines (CLSI 2014). PCR was ýperformed by specific primers of gyrA gene.ý
Hundred bacteria were isolated of clinical urine sample including 60 E.coli, 32 Klebsiella, 3 ýEnterobacter, and 5 Proteus. Antibiotic resistance to ciprofloxacin were 36%, nalidixic acid 45%, ýnorfloxacin 38%, ofloxacin 38%, levofloxacin 35% and enrofloaxin 39%. Totally, 36 bacteria were ýresist to all antibiotics, which 29 bacteria (80.55%) revealed mutation in gyrA gene. ý
This study revealed that Ecoli isolates carry a mutation in gyrA genes. This mutation has ýan important role in antibiotic resistance to quinolons.ý Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR.ý