Isolation and assessment of the pigments from the psychrotrophic bacteria with the aim of application in the food industries ‎

Nowadays, the use of the organic pigments produced by the microorganisms according ýto their nature and safety is increasing. These pigments are various in their nature and have different ýproperties. The aim of the recent research was to extract the new bacterial pigments that are produced ýin the cold environments. ý
Ten soil samples from different areas of Torghabeh city were collected and ýcultivated. Ten days after, 14 pigmented bacterial colonies were selected then the isolates were ýidentified using the biochemical tests and polymerase chain reaction (PCR). Using the methanol and ýglass powder, bacterial pigments were extracted and in order to purification TLC was performed. ýPigments of two strains, A8 and A13, which had a suitable purity, were used for cell toxicity using ýthree cell lines HepG2, human embryonic fibroblasts, and gastric cancer.
Among 14 pigmented bacterial strains, the strain A8 that was Aeromonas hydrophyla, could ýprevent the growth of the cells on different cancerous cell line. Also, the pigment that was named A13, ýhad no cytotoxic effects on all the used cell types and can be used as a natural coloring agent in the ýfood industry. ý
As the results indicate based on the biological properties of the extracted bacterial ýpigments, they can be used in different areas such as medicine and technology.ý Keywords: Psychrotrophic bacteria, Pigments, Food industry, Anti cancer effect.ý