Detection of ‎ cmlA/tetR, bla PSE-1, bla TEM and sip B in the ‎Salmonella strains by Multiplex-PCR method and their antibiotic ‎resistance pattern

Gastroenteritis due to Salmonella is common in human and considered as a global ýdilemma of public health. This study was done to determine cmlA/tetR, ýbla PSE-1, bla TEM and sip B ýin the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile. ý
In this cross-sectional study, 163 clinical samples were obtained from ýpatients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined ýusing the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for ýdetermination of these target genes by the specific oligonucleotides primers. ý
Of 163 collected samples, 48(29.4%) Salmonella spp., were obtained, which 25(52.1%) were ýS. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed ýthat the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and ýchloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, ýgentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of ýSalmonella spp., isolates carried cml/tetR and sipB genes, respectively. ý
According to our results, detection and genotyping of virulence genes and comparison ýwith global ranging is a basic requirement in the control and prevention of salmonellosis in industrial ýpurposes.ý Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR