Polymorphism of TFAM gene and its association with growth traits in Sistani cattle

Sistani beef cattle is a native breed of east of Iran that as a valuable genetic resource in the ýtropical area could be good a candidate to produce high quantity of muscularþ þcarcass portions ýwith highþ þefficiency. In order to explore the Mitochondrial transcription factor A gene ýý(TFAM) polymorphisms using PCR-RFLP, 150 Sistani cows were randomly selected and ýthen blood samples were taken from the jugular vein of each animal individually. ýThereafter, blood samples wereþ þsalting-out and electrophoresed with 1% agarose gel. The ýposition of the TFAM promoter region amplified by PCR and PCR products with 801bp ýlength were sliced byþ þBsuRI enzyme. Digestion products on 3% agarose gel were shown by ýelectrophoresis and staining with ethidium bromide. The patterns of digestion in AA ýhomozygotes, CC homozygotes, and AC heterozygotes were as follows: three bands with ýý152, 187, and 462bp; four bands with 83, 104, 152, and 462bp; and five bands with 83, 104, ýý152, 187, and 462bp, respectively. The results showed that the position of TFAM population ýdeviated from the Hardy-Weinberg equilibrium (P