Comparison and Optimization of Conjugated Linoleic Acid Production by Lactobacillus plantarum and Lactobacillus plantarum subsp. plantarum

Conjugated linoleic acid (CLA) was produced from castor oil using washed cells of Lactobacillus plantarum PTCC 1058 and Lactobacillus plantarum subsp. plantarum PTCC1745as the catalyst. Under the optimal reaction conditions washed cells of Lactobacillus plantarum PTCC1058 produced 1661.26mg CLA/L reaction mixture (36% yield of production) from 4.6mg/ml castor oil after 121h using 15% (w/v) cell. The resulting CLA was a mixture of two CLA isomers, cis-9, trans-11 (or trans-9, cis-11)-octadecadienoic acid (CLA1, 44% of total CLA) and trans-10, cis-12-octadecadienoic acid (CLA2, 46% of total CLA).The total production of CLA is extracellular in all of the reactions performed with Lactobacillus plantarum PTCC1058AlsoLactobacillus plantarum PTCC1745produced 1590.31mg CLA/L reaction mixture (16.5% production yield) from 9.6mg/ml castor oil after 121h using 15% (w/v) cell. Therefore Lactobacillus plantarum PTCC1058 was suggested for CLA production due to more production ability and CLA extracellular production.