Identification of Galbanum (Ferula gummosa) Seed Germination Inhibitor Factors and Inhibitory Effect of Galbanum Seed Extract on Germination and Growth of Amaranth and Wild Barley Weeds

To study themechanism of seed dormancy and increasing seed germination percentage and rate in galbanum, embryo, seeds with removed seed coat and intact seeds of this plant were firstly cultivated on MS medium. In the second experiment, the effect of some environmental factors (light and darkness, different temperatures including 8°C, 20°C and alternative temperature of 8°C and 20°C), GA3 and removed seed coat were studied on seed germination of galbanum. In the third experiment the effect of aqueous extract of galbanum seed were studied on seed germination and growth of Amaranth and Wild barley. On the basis of results, 95% of galbanum embryos were grown on MS medium normally. Low temperature (8°C) and removing seed coat had significant effects on seed germination percentage and seed germination rate of galbanum, so that seeds with removed seed coat were germinated 77% at 8°C temperature. Pretreatment of seeds with GA3 had no significant effect on seed germination percentage but increased seed germination rate. Also, darkness treatment had no effect on seed germination of galbanum. All different concentrations (2.5%, 5% and 10%) of galbanum seed aqueous extract had significant inhibitor effect on the seed germination and growth of amaranth and wild barley. Results showed that galbanum seed dormancy could be studied in two plant, physiological dormancy that can be removed by low temperature and chemical dormancy which can be overcomed by removing seeds coat.