Antiproliferation Effect of Heat Killed Indigenous Probiotic Bacteria on AGS Cell Line

Aopoptosis induction is one of the best method cancer therapy and funding the anti cancer component¡ especially inducing opoptosis is of the significant important. This study aimed to assess cytotoxic effects of heat-killed probiotic bacteria and induced apoptosis on gastric cancer AGS cells compared to normal cells (HEK-293). AGS cancer cell line AGS was cultured in RPMI medium¡ and cell line HEK-239 was selected as the control. Both cell lines received 0.01¡ 0.1¡ 1¡ 10¡ 100¡ and 1000 µg/ml of heat-killed lactobacillus paracasei for periods of 24¡ 48¡ and 72 hours. Viability of cells was measured using MTT assay. Apoptosis assay was performed in IC50 of cell culture by Annexin V and propidium iodide staining methods in 48 hours. According to MTT assay¡ heat-killed bacteria reduced viability and proliferation of gastric cancer cell line AGS in pattern of time¡ dose and strain dependent. The highest cytotoxic effect was observed in 1000 µg/ml for 72 hours. Annexin V staining and flow cytometry confirmed induced apoptosis in AGS cell line in pattern of time¡ dose and strain dependent. Heat-killed probiotic bacteria showed cytotoxic and induced apoptosis effects on AGS gastric cancer cell line¡ with slight cytotoxic effect on HEK-239 compared to AGS. Further studies are still required to confirm these bacteria as a biological anti-cancer product in treatment and prevention.